ejemplo markdown

ExamIDs2026a

Trabajo Fuentes de la Información

Follow-up from our first meeting with guidance on getting started with GitHub Desktop, Rprojects, and the swirl tutorial

This pipeline processes barcode counts sequenced from twelve fluorescence-activated cell sorting (FACS) samples of the synTCS-MutLib strain in the fumarate condition. Sequence data was generated using the Illumina NextSeq platform using paired-end sequencing read amplicons. Raw sequencing data was pre-processed on a high-performance computer using the Makefile script available in the project GitHub repository. Here, pre-processed barcode-count files for all twelve fumarate samples are merged, adjusted abundances are computed using the protocol from Biswas et al., (2021), and a median activation score- here, "median bin"- is calculated for each observed barcode. This analysis is replicated for the No Ligand and Aspartate samples.

This pipeline processes barcode-sequence-phenotype data previously generated in Merge_and_MEFL_Convert.Rmd and determines which multi-barcoded mutnts have significant differences between their fold-change scores.