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Project Basic Statistics
Assignment 2 - NGUYEN THI MY DIEU
Who is Dieu Nguyen
Sample exploration and differential expression: PCA and DESeq2
In this practical session, we continue working with the GSE161731 blood RNA-seq dataset from Lab 6 to investigate a biological question: **which genes are switched on or off in the blood of COVID-19 patients compared with healthy volunteers?** We will first use **principal component analysis (PCA)** to explore similarities and differences between samples and assess whether disease status is a major source of variation in the data. We will then use **DESeq2** to identify genes that are differentially expressed between COVID-19 and healthy samples, account for multiple testing across thousands of genes, and visualise the results using a **volcano plot, individual gene-expression plots, and a heatmap**.
Actividad_del_1er_50
Actividad_del_1er_50
