This pipeline processes and visualizes barcode-sequence-phenotype data previously generated in Merge_and_MEFL_Convert.Rmd.

This pipeline processes barcode counts sequenced from twelve fluroescence-activated cell sorting (FACS) samples of the synTCS-MutLib strain in the aspartate condition. Sequence data was generated using the Illumina NextSeq platform using paired-end sequencing read amplicons. Raw sequencing data was pre-processed on a high-performance computer using the Makefile script available in the project GitHub repository. Here, pre-processed barcode-count files for all twelve aspartate samples are merged, adjusted abundances are computed using the protocol from Biswas et al., (2021), and a median activation score- here, "median bin"- is calculated for each observed barcode. This analysis is replicated for the No Ligand and Fumarate samples.

This pipeline processes barcode counts sequenced from twelve fluroescence-activated cell sorting (FACS) samples of the synTCS-MutLib strain in the no ligand condition. Sequence data was generated using the Illumina NextSeq platform using paired-end sequencing read amplicons. Raw sequencing data was pre-processed on a high-performance computer using the Makefile script available in the project GitHub repository. Here, pre-processed barcode-count files for all twelve no ligand samples are merged, adjusted abundances are computed using the protocol from Biswas et al., (2021), and a median activation score- here, "median bin"- is calculated for each observed barcode. This analysis is replicated for the Fumarate and Aspartate samples.

Mapa

logistic regression

esta ´padre

Este proyecto desarrolla un análisis exploratorio y cuantitativo sobre los procesos de liquidación económica del uso de fauna silvestre nativa en Colombia, bajo el marco de la Ley 99 de 1993. Utilizando técnicas de ciencia de datos y estadística multivariada en R, el estudio aborda la complejidad de los sistemas de información ambiental para identificar patrones, sesgos y relaciones clave en el cálculo de tasas compensatorias.

This pipeline processes barcode-sequence-phenotype data previously generated in Merge_and_MEFL_Convert.Rmd and determines positions which are significantly enriched for influencing aspartate responsiveness and specificity based on the total number of barcodes observed.

mapa final